electric cell substrate impedance sensing  (Applied BioPhysics)

Journal: bioRxiv

Article Title: Direct effects on endothelial cells are essential for perivascular cell (pericyte)-dependent amplification of orthoflavivirus NS1-mediated microvascular leakage

doi: 10.64898/2026.01.29.702573

Figure Lengend Snippet: (A-B) ECIS measurement of permeability of monocultures of HUVECs (A) or LSECs (B) treated with preconditioned media from HSCs treated with 1 µg/mL NS1 or 100 ng/mL TNF-α for 24 h; “Untreated” refers to HUVECs or LSECs treated with HSC media only and the “EC only” refers to untreated HUVECs or LSECs without the addition of HSC media. (C, D) Dextran permeability assay measuring HUVEC (C) or LSEC (D) permeability in coculture with HSCs 24 h post-treatment with 1 µg/mL NS1, 100 ng/mL TNF-α (positive control), or eluate (negative) control. Data points represent technical replicates within an experiment with different symbol shapes corresponding to different experiments (N=3, n=3). Bars represent the mean, and error bars represent SEM. (E, F) TEER measurement of semi-contact cocultures of HUVECs (A) or LSECs (B) cultured with HSCs and treated with 1 µg/mL NS1, 100 ng/mL TNF-α (positive control), or eluate (negative) control. Permeability measurements were normalised to each sample pre-treatment for ECIS data (A, B) or the untreated endothelial cell only control (EC only; dashed line) for EVOM data (E, F). In the time course experiments (A, B, E, F), each data point represents the mean ± SEM. In all experiments indicated significance is compared to untreated cocultures; * P < 0.05; ** P < 0.01; *** P < 0.001.

Article Snippet: For the transendothelial electrical resistance (TEER) assay using the Electric Cell-substrate Electrical Impedance Sensing (ECIS) Z-Theta system (Applied Biophysics, Troy, NY USA), 8W10E+ arrays were pre-treated overnight at 37 °C with 400 μL of culture media prior to coating with 0.5 μg/mL human fibronectin (Merck) for HUVECs or 15 μg/mL bovine fibronectin (Innoprot) for LSECs.

Techniques: Permeability, FITC-Dextran Permeability Assay, Positive Control, Negative Control, Cell Culture, Control

Journal: bioRxiv

Article Title: Direct effects on endothelial cells are essential for perivascular cell (pericyte)-dependent amplification of orthoflavivirus NS1-mediated microvascular leakage

doi: 10.64898/2026.01.29.702573

Figure Lengend Snippet: Endothelial barrier integrity measurements for HUVEC (A, B) or LSEC (C) monocultures following treatment with 1 µg/mL wild-type DENV-2, AHFV or KFDV NS1, or the nonfunctional DENV-2 NS207Q mutant (negative control), or 100 ng/mL TNF-α (positive control), or eluate (negative) control as indicated. Permeability measurements were normalised to the untreated endothelial cell only control (Untreated) for EVOM TEER data (A), or to each sample before treatment for ECIS data (B, C). Each data point represents the mean ± SEM. Indicated significance is for treatment versus untreated EC only control (Untreated). * P < 0.05; ** P < 0.01; *** P < 0.001.

Article Snippet: For the transendothelial electrical resistance (TEER) assay using the Electric Cell-substrate Electrical Impedance Sensing (ECIS) Z-Theta system (Applied Biophysics, Troy, NY USA), 8W10E+ arrays were pre-treated overnight at 37 °C with 400 μL of culture media prior to coating with 0.5 μg/mL human fibronectin (Merck) for HUVECs or 15 μg/mL bovine fibronectin (Innoprot) for LSECs.

Techniques: Mutagenesis, Negative Control, Positive Control, Permeability, Control